Hi,
No, There is no setup fee is being charged for using this service, and at the end of month Amazon.com will charge you for the uses of web Services.
You can view your charges for the current billing period on the Amazon Web Services web site.
for more detail go through following link -
http://www.sdbexplorer.com/documentation/simpledb--how-to-get-simpledb-account-activity-detail.htmlRegards-
Udit